This is a image of cheek cells under 40X magnification.
The Microscope
History of the Microscope
The first microscope made was by Hans and Zacharias Janssen around 1595in Middleburg, Holland. The compound microscope that they made was a tube with lenses at both ends. The magnification range of this scope ranged from 3X to 9X.
Robert Hooke made and improvement to the compound microscope around 1600. He discovered the features of plant tissue under the microscope and called his findings plant cells.
Anton van Leeuwenhoek built the best simple microscopes in his time 1632-1723. He was the fist person to describe bacteria, and helped to prove the theory of blood circulation.
Types of Microscopes
There are four types of microscopes: The Compound Microscope, Dissection Microscope, Scanning Electron Microscope, and the Transmission electron Microscope. The compound microscope is light illuminated. The image seen is two-dimensional. It is the most commonly used out of the different microscopes. It has a high magnification and a low resolution. The dissection microscope is light illuminated. The image seen is 3-dimensional. It has a low magnification; you are not able to see individual cells. The scanning electron microscope uses electron illumination. The image seen is 3-dimensional. It has high magnification and resolution. The transmission electron microscope is electron illuminated. The image seen is 2-dimensional. It has high magnification and resolution.
How to Use a Microscope
1. Turn on the light
2. Adjust the rheostat dial to 10; the dial moves left and right.
3. Adjust the iris diaphragm to change the amount of light coming through the aperture, the iris lever moves left to right.
4. Put your slide on the stage using the stage clips.
5. Make sure the 4X lens is in place; the dial moves left and right.
6. Move the stage left and right and up and down until the specimen is centered over the aperture.
7. Use the course focus dial to move the stage as high as it will go, turn the dial up.
8. After you have done these steps you now need to look through the oculars.
9. You need to adjust the oculars until the 2 circles of light are one circle.
10. You will then need to adjust the coarse focus dial down until you see the specimen (only use the coarse dial when on low powered scope)
11. After you have adjusted the coarse dial and see the specimen, adjust the fine focus knob to sharpen the image.
12. Re-center the specimen if needed.
13. Adjust the iris diaphragm to adjust the amount of light.
14. Once the specimen is in focus you can then move to the next higher-powered lens.
15. You will then repeat steps 11-13 to get the specimen in focus.
16. Repeat steps 11-15 for all remaining higher-powered lenses.
1 comment:
I am often asked which microscope is the best choice for laboratories who require high quality at an affordable price without sacrificing optical clarity.
The Nikon Eclipse 200 is the cream of the crop, with regards to the under $2,000.00 microscope class for clinical laboratories. It comes pre-configured with 4x, 10x, 40x, and 100x oil PLAN objectives. The eyepieces are 10x with a 20mm field of view. The optics are amazing. The quality of the microscope is tops. We've been in the microscope service business since 1969, and have serviced every brand, make, and model of microscope out there, and I can honestly say, that the Nikon E 200 is the best clinical microscope for end uses who do not require a Pathology grade microscope like the Nikon 50i
The Nikon Nikon E200 is also fully upgradeable to a fluorescent unit. It's also available in a trinocular version for people interested in digital imaging of specimens. Polarization accessories, as well as a teaching head (side by side, and face to face) configuration are also available.
The good thing with Nikon is, you never have to pay retail, and the customer service is the class of the industry.
Find pricing on Nikon Microscopes by clicking --> Nikon Microscope
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